KMID : 0355620080340060635
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Journal of Korean Association of Oral and Maxillofacial Surgeons 2008 Volume.34 No. 6 p.635 ~ p.639
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EFFECT OF DIFFERENT STERILIZATION METHODS ON THE SURFACE MORPHOLOGY OF PPDO-hybrid-PLGA NANOFIBER SCAFFOLD AND ATTACHMENTS OF PC12 CELL
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Lee Ju-Hyon
Min Hyun-Gi Jeong Ju-Young Kang Na-Ra
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Abstract
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Objectives: the effect of different sterilization methods on the surface morphology of PPDO-hybrid-PLGA nanofiber scaffold and attachments of PC12 cell were investigated.
Methods: Poly (p-dioxone)-hybrid-Poly (lactide-glycolide) (PPDO-hybrid-PLGA) nanofiber scaffold, fabricated in a tube form with 1.5 mm internal diameter, 0.2 mm thickness and 5 mm length, was prepared using electrospinning method. To study the surface morphology using SEM, The study group and control group in respective were; Control:Non-sterilized scaffold, Group I:scaffold sterilized with 70% Alcohol, Group II: scaffold sterilized with Ethylene Oxide at 65 ¡É , and Group ¥²: scaffold sterilized with Ethylene Oxide at 37 ¡É. To investigate viability of the PC12 cell on the scaffold, The study group and control group in respective were; Control: sterilized with 70% Alcohol, Group I: sterilized with Ethylene Oxide at 65 ¡É, and Group II: sterilized with Ethylene Oxide at 37 ¡É.
Results: 1. The surface morphology was slightly changed in Group I, II and Group¥², compared with control.
2. The attachment of PC12 cells in Group I, II was not higher than in control
Discussion: The attachment of PC12 cell is not influenced by different sterilization methods.
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KEYWORD
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Sterilization, Nanofiber scaffold, Surface morphology, Attachment of cell
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